Maple Syrup Urine Disease (MSUD), also called branched-chain ketoaciduria, is an autosomal recessive metabolic disorder affecting branched-chain amino acids (BCAAs). Screening is conducted in the Philippines to detect MSU in newborns. This article discusses experimental research utilizing bacterial inhibition assay, specifically Escherichia coli MCC-MNH 1958, to detect excess BCAAs.

Learning Objectives:

• Describe bacterial inhibition assay and the procedure
• Explain the uses of the bacterial inhibition assay
• Discuss the potential of Escherichia coli to detect Maple Syrup Urine Disease

Author(s):

• Jon Paul M. Reyes, PhD, RMT, AHI (AMT), MSMLS, MBA, CHRA, DPed
• Oliver Shane R. Dumaoal, RMT, MSMT, CBO, IFBA PC

Relevant Certifications:

• Allied Health Instructor (AHI)
• Medical Laboratory Technologist (MLT)
• Medical Laboratory Scientist (MLS)
• Phlebotomy Technician (RPT)

Launch Date: 2023

Enrollment Expiration: 180 days after purchase